Kingfisher Biotech, Inc. is committed to accelerating animal health research and human disease research done in animal models by developing and commercializing research reagents specific for a variety of species, including bovine, canine, chicken, dolphin, equine, feline, ferret, guinea pig, hamster, human, goat, monkey, mouse, ovine, rabbit, rat, salmon, swine, and zebrafish. We will continue to add species as our customer request specific reagents for their research. Currently, we offer over 2000 recombinant proteins, polyclonal antibodies, monoclonal antibodies and ELISA kits for animal health and animal model research. Our products are primarily cytokines, chemokines, interferons, and growth factors.

 

Our proteins are expressed using a yeast expression system. Yeast has several advantages as an expression system, and the recombinant proteins generated by yeast have several characteristics that make them ideal for many applications including cell culture. The yeast expression system used by Kingfisher Biotech secretes recombinant protein into the media. This secretion is typically considered the first step in the purification process because yeast cells inherently secrete very few native proteins. Recombinant proteins secreted by yeast are processed by a Golgi apparatus, thus they may be post-translationally modified. These modifications can include glycosylation, phosphorylation and sulfation. There are many proteins that require modifications for protein function, proper folding or solubility. Another added benefit of a yeast expression system is that yeast do not have a bacterial cell wall like E. coli, thus there is no endotoxin (lipopolysaccharide) present. Yeast are naturally endotoxin-free.

 

Our polyclonal antibodies are antigen affinity purified. To make them even more specific, we perform a proprietary purification step. We provide a graph with all our polyclonal antibodies showing the customer exactly how the antibody performs. This takes the guess work out of working with our antibodies.

 

The Do-It-Yourself ELISA kits are the stand-alone unconjugated antibody (capture antibody), recombinant protein (standard), and biotinylated antibody (detection antibody). Each of these components can be bought individually, if desired (or as components run out). These antibodies have been shown to function together with the standard provided in an ELISA. Evaluating the individual components’ Data Sheet will give a representative working concentration to help determine the number of assays that can be performed.